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AAT Bioquest

FAQ

Posted on March 22, 2023
What are the applications of Ziehl-Neelsen staining?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the reagents used in the Ziehl-Neelsen stain?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the principle of the Kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the procedures of the kinyoun-cold method?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the limitations of the Kinyoun-cold method?
FluorescenceGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What's the role of iodine in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What is the role of alcohol in the Gram stain procedure?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the fundamental differences between Ziehl-Neelsen technique and the Kinyoun technique?
FluorescenceGram StainingGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 22, 2023
What are the types of chemical reagents used in the staining process of the tissues?
Cellular Structures and OrganellesFluorescenceImmunohistochemistry (IHC)Organelle Stainingfluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What toxins are produced by gram negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 2, 2023
What is the composition of the cell envelope?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
How do I distinguish gram negative and gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-negative cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What are the components of gram-positive bacteria cell walls?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What infections are caused by gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Is it easier to kill gram-positive bacteria than gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
Which is more harmful, gram-positive bacteria or gram-negative bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on March 1, 2023
Where are gram-positive bacteria found?
FluorescenceGram StainingGram-negativeGram-positivefluorescence imagingfluorescence microscope


Posted on March 1, 2023
What is the significance of gram-positive bacteria?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on January 13, 2023
What are the differences between chemiluminescence and electrochemiluminescence?
D-LuciferinEnzymesFluorescenceLuciferaseschemiluminescencefluorescence imagingluminescense


Posted on January 13, 2023
What are the differences between bioluminescence and fluorescence?
Bioconjugation Chemical ReagentsEnzymesFluorescenceLuciferasefluorescence imagingluminescense


Posted on January 13, 2023
What are the types of molecules that exhibit fluorescence?
Bioconjugation Chemical ReagentsCoumarinsCyaninesFluoresceinsFluorescenceRhodamines and Rhodamine Derivativesfluorescence imaging


Posted on January 13, 2023
What should I consider when choosing a fluorescence spectrometer?
Chemical ReagentsFluorescenceSpectral Flow Cytometryemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on January 13, 2023
What are the different types of spectrometers used in fluorometry?
Bioconjugation Chemical ReagentsFluorescenceemission wavelengthexcitation wavelengthfluorescence imagingspectrophotometer


Posted on August 3, 2021
Do you have any dyes that can stain the nuclei in live cells? Are they also fixable and can I use them to stain fixed cells?
Nuclei StainingDNAFluorescenceemission wavelengthfixablefixed cellsfluorescence imaginglive cell analysisnuclear DNAMicroscopy


Posted on June 1, 2020
What is the difference between chemiluminescence and fluorescence?
FluorescenceLuciferasechemiluminescencefluorescence imagingluciferin


Posted on June 1, 2020
What are the common methods for bacteria counting?
Flow Cytometry ReagentsFluorescenceGram Stainingbacteriaflow cytometryfluorescence imagingfluorescence microscope


Posted on June 1, 2020
Are acid-fast bacteria gram positive or gram negative?
FluorescenceGram StainingGram-negativeGram-positivebacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What are acid-fast bacteria?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on June 1, 2020
What is the difference between bacteria and viruses?
FluorescenceGram Stainingbacteriafluorescence imagingfluorescence microscope


Posted on May 9, 2020
What are oligonucleotides?
Förster Resonance Energy Transfer (FRET) Peptide and Oligonucleotide LabelingDNA and RNAFRETPeptide and Oligonucleotidefluorescence imaging


Posted on February 19, 2020
Why is my Fluo-4 signal not at a useful magnitude?
Calcium dyescalcium indicatorDissociation Constant (Kd)cell-permeable probesfluorescence imaging


Posted on December 12, 2019
What is Stokes Shift?
Stokes ShiftFluorescenceemission wavelengthexcitation wavelengthfluorescence imaging


Posted on December 11, 2019
Can I Gram stain living cells?
Cell AssayCell CytotoxicityFixationGram stainingbacteriafluorescence imaginglive cell assay


Posted on December 6, 2019
What is cd80 and cd86?
Cell Plasma Membrane probesMembrane MarkerPlasma membrane stainingantibody labelingfluorescence imagingT cell


Posted on November 1, 2019
What is fluorescence crosstalk?
iFluor dyesControlscompensation correctioncorrection factordual stainsemission wavelengthexcitation wavelengthfluorescence imagingcrosstalk


Posted on September 16, 2019
Why are cells washed?
bufferfluorescence imagingimmunofluorescencelive cell analysisno washcell cultureisoelectric point isotonic


Posted on August 21, 2019
Can I measure NADPH without lysing my cells?
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex


Posted on June 14, 2019
What does TR-FRET mean?
Fluorescence Quantum Yieldemission wavelengthexcitation wavelengthfluorescence imagingautofluorescenceFRETTerbiumEuropium


Posted on June 13, 2019
Which ROS reagent is best for use in small animals such as mice or rats? What is the recommended dosage?
ROSfluorimetricin vivo imagingfluorescence imaging


Posted on June 13, 2019
What is the mechanism of your Cell Meter™ Autophagy Assay Kits (cat#23000, 23001 and 23002)
Autophagylysosomefluorescence imagingfluorescence microscope


Posted on June 13, 2019
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
anti-fadingfluoresceinfluorescence imagingfluorescent microscope


Posted on June 13, 2019
Do your autophagy assay kits be used with a multi-function microplate reader to detect fluorescence intensity and reflect autophagy levels?
Autophagyfluorescence imagingfluorescence microscopeflow cytometer


Posted on June 13, 2019
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
Calcium dyescalcium concentrationcalcium indicatorfluorescence imagingfluorescent calcium indicatorcalbryte 520


Posted on June 12, 2019
Do you have fixable live cell staining assay kits?
Cell CytotoxicityCell ViabilityMultidrug Resistance (MDR1/P-gp, MRP1)flow cytometryfluorescence imagingfluorescence microplate readerfluorescent microscopefluorescent plate readerlive cell assay


Posted on April 2, 2019
Can I treat my cells before adding the master reaction mix for ROS kit 22900
ROSfluorescence imagingfluorescent microscopefluorescence microscope


Posted on April 2, 2019
Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?
flow cytometerflow cytometryfluorescence imagingfluorescence microplate readerfluorescence microscopefluorescent plate reader


Posted on April 2, 2019
Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
Actin iFluor dyesCell AssayFixationF-actinFixativesfluorescence imagingfluorescence microscopephalloidin


Posted on January 3, 2019
What is the number of passages your Cell Explorer Live Cell Tracking assays can undergo?
fixableflow cytometerlive cell trackingfluorescence imagingfluorescent microscope


Posted on January 3, 2019
Do you have any fixable mitochondria staining assay kits?
fixablemultiplexmitochondriafluorescence imagingfluorescence microscope


Posted on September 27, 2018
How do I stain T cells with CytoTell™ Blue?
Autophagylysosomefluorescence imagingfluorescence microscopecell proliferation


Posted on August 30, 2018
How do you stain paraffin-embedded tissue with a phalloidin"conjugate?
Phalloidinfluorescence imagingfluorescence microscope


Posted on July 8, 2018
What are the differences between your fluorimetric peroxynitrite assays (Cat# 16315, 16316 and 16317)?
ROSCell Assayflow cytometerperoxynitrite anionfluorescence imagingfluorescent microscope


Posted on March 8, 2018
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
DNARNAdual stainsnucleic acidfluorescence imaging